FastQCFastQC Report
Wed 1 Nov 2017
HVY3KBCXY_l01n02_chip_ctrl_replicate_2_shoot_h3k27ac_09_13_17.352000000a5981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVY3KBCXY_l01n02_chip_ctrl_replicate_2_shoot_h3k27ac_09_13_17.352000000a5981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3283269
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC3152.8921932E-1012.14292841
GTTAACG3602.6575435E-910.62506140
ACGCAGG4956.1118044E-109.09068444
GGTTAAC5251.6771082E-88.14265539
CGTAAAC3603.0538307E-58.1251720
CTCGTAA4001.1331432E-47.31220818
TAACGCA4751.682269E-57.104979542
CGTGTGC6356.334085E-87.08600761
CTTCCGA5901.0111526E-66.86455069
AACGGGA6001.336788E-66.749421616
CAGGTTA6456.2142317E-76.627743737
AACGCAG5958.425583E-66.428412443
ACTCGTA4200.00156569246.42821717
TCCGATC8303.1130185E-86.23459611
TTCCGAT6151.4059773E-56.219168710
AAACGGG5905.1210533E-56.101358415
CGCAGGA7052.8609793E-66.063679745
CCACGAT6708.104198E-66.04471926
TTTCGGG6402.5931904E-55.97650587
GTGTGCT8452.8926115E-75.85748622
Produced by FastQC (version 0.11.2)