FastQCFastQC Report
Wed 1 Nov 2017
HVY3KBCXY_l01n01_chip_ctrl_replicate_2_shoot_h3k27ac_09_13_17.351000000a5984.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVY3KBCXY_l01n01_chip_ctrl_replicate_2_shoot_h3k27ac_09_13_17.351000000a5984.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3283269
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAA4101.6370905E-1110.97541618
CGTAAAC3552.1100277E-910.77462220
ACTCGTA4854.0672603E-98.81427817
TCGTAAA4955.362199E-88.18179919
GGTTAAC3704.302927E-57.90526739
GTTAACG2950.0024403247.62698440
TAACGCA3000.002877237.499868442
TACTCGT5301.3777553E-67.21685416
ATACTCG4801.9476443E-57.031126515
GACGTTT5552.8444229E-66.891875345
CTGACGT5603.273637E-66.830341343
GCAATAC6601.2810233E-76.81806212
GTAAACC6101.7525035E-66.639328521
TGACGTT6252.6110538E-66.479984844
TCGCTGA6901.9816143E-66.19554340
CGCTGAC8302.038687E-75.963750441
CTCGCTT7001.6410948E-55.7860532
ACGTCTG7952.1670892E-55.377345645
CTTTTCG8955.1143816E-65.27939755
TCGGGAA6500.00123127715.19221649
Produced by FastQC (version 0.11.2)