FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28375239
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7404412.609461721185855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGCG2700.046.6761568
CGGCCCG5300.046.2336152
GTACTCT12600.041.106133
TACTCTA13800.038.5418434
GGCCCGC5800.038.02416253
TCTAGTT13900.037.257017
ACTCTAG19350.033.8164945
CGCGGTC1055.511538E-1033.3407570
CGGGCCC5250.033.33534662
CGCGCCG6800.031.91577357
GCACACG4550.031.53102911
CTCTAGT17950.031.3854716
TGTACTC16450.031.273632
GTTACCT16550.030.86852814
TTGTACT16550.030.6910651
CACACGT5000.030.09285212
TGTGCGG2450.029.99472635
TGTTACC17250.029.81900613
GCCCCCC3650.028.7665865
ACGTCTG5000.028.6928815
Produced by FastQC (version 0.11.5)