FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_9R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_9R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753710
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA78020.44488541435014917No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG34610.19735304012636068No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT33040.18840059074761506No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA31300.17847876786926004No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT30750.1753425594881708No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT25290.14420856355953948No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA24350.13884849832640517No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG24020.13696677329775164No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA22610.12892667544805014No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA21770.12413682992056839No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC21600.123167456420959No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT21310.12151381927456649No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG21000.11974613818704347No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG17600.10035866819485548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA18100.046.597814
TGTACTC18650.045.0359572
TTGTACT19450.043.184811
GTACTCT19750.042.7048263
ACTCTAG24550.041.340295
TCTAGTT20750.040.982917
CTCTAGT21400.039.4110376
CGTATGC3200.038.28745744
CTAGTTG23600.035.8864368
GTATGCC3350.035.5281545
TATGCCG3200.035.00467746
GCACACG3350.032.38511
TAGTTGT26650.031.516729
TGTTACC27150.030.93630213
GTTACCT27800.030.21297314
ACCTCTA27950.029.9273217
TACCTCT28200.029.9076716
CTAATGC28150.029.47107921
TTACCTC29000.029.20330415
TCTAATG29300.028.55004720
Produced by FastQC (version 0.11.5)