FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_9G-ABF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_9G-ABF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077618
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA74290.3575729513317655No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC52440.25240443623418746TruSeq Adapter, Index 3 (97% over 36bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG36770.1769815240337733No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT31500.15161593709719495No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA30940.1489205426599115No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT26430.127212991031075No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT25820.12427693637617696No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA23240.11185886914726385No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG21390.10295444109552382No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA21110.10160674387688208No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA20880.10049970687585495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5950.056.47015846
CGTATGC6100.055.08155444
GTATGCC6100.055.08155445
GCACACG6550.052.36475811
CTCGTAT6450.051.5542
TCGTATG6600.050.3784143
ACGTCTG6750.050.29349515
CACGTCT6900.049.20015714
AGCACAC7050.049.1473810
CACACGT7050.048.15450312
GCCGTCT7300.046.50538349
GTCACTC7200.046.18242629
GTACTCT18400.045.6529273
TACTCTA18850.045.3057864
CAGTCAC7350.045.2399327
CATCTCG7450.045.1003339
GAGCACA7850.044.141929
TCTAGTT19950.042.2824177
AGTCACT7950.041.82559228
TCCAGTC8400.041.25167525