Basic Statistics
Measure | Value |
---|---|
Filename | HVWNMBGXB_n01_9G-ABF3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2077618 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 7429 | 0.3575729513317655 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC | 5244 | 0.25240443623418746 | TruSeq Adapter, Index 3 (97% over 36bp) |
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG | 3677 | 0.1769815240337733 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 3150 | 0.15161593709719495 | No Hit |
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA | 3094 | 0.1489205426599115 | No Hit |
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT | 2643 | 0.127212991031075 | No Hit |
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT | 2582 | 0.12427693637617696 | No Hit |
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA | 2324 | 0.11185886914726385 | No Hit |
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG | 2139 | 0.10295444109552382 | No Hit |
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 2111 | 0.10160674387688208 | No Hit |
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA | 2088 | 0.10049970687585495 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 595 | 0.0 | 56.470158 | 46 |
CGTATGC | 610 | 0.0 | 55.081554 | 44 |
GTATGCC | 610 | 0.0 | 55.081554 | 45 |
GCACACG | 655 | 0.0 | 52.364758 | 11 |
CTCGTAT | 645 | 0.0 | 51.55 | 42 |
TCGTATG | 660 | 0.0 | 50.37841 | 43 |
ACGTCTG | 675 | 0.0 | 50.293495 | 15 |
CACGTCT | 690 | 0.0 | 49.200157 | 14 |
AGCACAC | 705 | 0.0 | 49.14738 | 10 |
CACACGT | 705 | 0.0 | 48.154503 | 12 |
GCCGTCT | 730 | 0.0 | 46.505383 | 49 |
GTCACTC | 720 | 0.0 | 46.182426 | 29 |
GTACTCT | 1840 | 0.0 | 45.652927 | 3 |
TACTCTA | 1885 | 0.0 | 45.305786 | 4 |
CAGTCAC | 735 | 0.0 | 45.23993 | 27 |
CATCTCG | 745 | 0.0 | 45.10033 | 39 |
GAGCACA | 785 | 0.0 | 44.14192 | 9 |
TCTAGTT | 1995 | 0.0 | 42.282417 | 7 |
AGTCACT | 795 | 0.0 | 41.825592 | 28 |
TCCAGTC | 840 | 0.0 | 41.251675 | 25 |