FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_8G-ZAT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_8G-ZAT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214400
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC45770.2066925578034682TruSeq Adapter, Index 9 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA36830.16632044797687862No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT27550.12441293352601156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5650.054.51816644
TATGCCG5700.053.4270546
GTATGCC5950.051.1810645
GCACACG6250.048.71666711
CACACGT6650.045.78427512
TCGTATG7000.044.0049443
ACGTCTG6950.043.80896415
CTCGTAT7250.043.4511942
CACTATA7200.042.2897331
AGCACAC7650.040.25868610
TATCTCG8250.036.909939
CAGTCAC8450.036.8672127
TACTCTA12100.036.7297064
CACGTCT8400.036.66250214
AGTCACT8650.036.0123528
GGCCCCT501.7125913E-434.9944461
GTACTCT12600.033.8835073
GAGCACA9600.032.444969
GCCGTCT9300.032.36849649
CTATAAT9800.031.7806733
Produced by FastQC (version 0.11.5)