FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_7G-ABF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_7G-ABF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227908
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA52500.23564707339800384No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGC37110.16656881702476045TruSeq Adapter, Index 7 (97% over 35bp)
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA29560.1326805236122856No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA25210.11315548038787956No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA25030.1123475475648007No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT24670.11073168191864295No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT24600.11041748582077897No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG23730.10651247717589775No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT23130.10381936776563486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4900.055.71475244
TATGCCG4850.055.5699746
GTATGCC5000.053.90165745
AGCACAC5550.049.1873910
CACTACA5550.049.18628731
TCGTATG5550.048.56005543
GTACTCT13100.048.3570143
GCACACG5600.048.12323811
CACACGT5700.047.27897312
CTCGTAT5750.046.26125342
CTACAGC5900.045.67424833
ACGTCTG5950.045.2924615
TACTCTA13950.045.159644
CACGTCT6000.044.91502414
CATCTCG6100.044.7544739
TCTAGTT14250.044.2069247
ACTACAG6300.042.77525332
TACAGCA6450.041.78048334
GAGCACA6800.040.6593639
CAGTCAC6800.040.14649227