FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_6R-MYB44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_6R-MYB44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349890
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA206421.5291616353925135No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT100660.7456903895872997No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA92060.6819814947884643No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG80450.5959744868100364No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT70180.5198942136025899No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT66590.49329945402958764No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA64300.47633510878664187No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA63210.4682603767714406No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA60060.44492514204861133No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC57110.4230715095304062TruSeq Adapter, Index 7 (97% over 35bp)
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC46160.34195378882723776No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG44290.3281008082140026No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT37160.27528168961915417No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA35650.26409559297424234No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG32060.23750083340124012No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT29690.21994384727644475No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG28400.21038751305661943No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT27490.20364622302557986No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT25940.19216380593974322No Hit
ATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTA25650.1900154827430383No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC25510.18897836119980146No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC25050.18557067612916608No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG23390.17327337783078622No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG23140.1714213750750061No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC23070.1709028143033877No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATA22520.16682840824067147No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG22380.16579128669743462No Hit
GTTTGGTACTATCTGCTATGTATCCTGTCCTACTATCTAACCTCTGTAGG21510.15934631710731986No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA21190.15697575357992133No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAG20650.15297542762743632No Hit
GCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCT20430.15134566520234982No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC20110.1489751016749513No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC19620.1453451762736223No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG17090.12660290838512767No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT16680.12356562386564832No Hit
AAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAG16600.12297298298379869No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT16570.12275074265310505No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT16300.12075057967686256No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAG15480.11467601063790384No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA15130.11208320677981169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15070.11163872611842447No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT14560.10786064049663306No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT14530.10763840016593945No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA14250.10556415707946573No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA14210.10526783663854092No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC14150.1048233559771537No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA13970.10348991399299202No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGG13850.10260095267021759No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT13730.10171199134744312No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGG13620.10089711013489988No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA13610.10082303002466868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6350.066.6941842
CGTATGC6500.066.23939544
GTATGCC6650.065.2692345
TCGTATG6650.064.7452743
TATGCCG6750.064.30228446
CTCAATC6700.063.72783736
GCCGTCT6850.063.3612249
AAAAAGG6800.063.30299869
AGTCACC6850.062.85024328
CTGCTTG7050.062.0579257
CCACTCA7000.061.99429333
CAATCTC6900.061.37798338
ATGCCGT7150.061.1945147
TCCAGTC7100.061.12792625
ACCACTC7100.061.12792632
CACCACT7100.061.12792631
ACTCAAT7050.061.06046735
CAGTCAC7000.061.00342627
ACTCCAG7150.060.2131723
CCAGTCA7250.058.89985326
Produced by FastQC (version 0.11.5)