FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_6G-HB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_6G-HB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161155
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA106820.4942727384199652No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC66920.3096492384859022TruSeq Adapter, Index 3 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT48550.22464839403004414No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA46560.21544035481027507No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG45750.211692358946952No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT38090.17624834868392134No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT37350.17282425369767554No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA33720.15602767964352396No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA30560.1414058686211771No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA29980.13872211849682228No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG27740.12835729043034858No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC27140.1255809971982574No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT22290.1031392935721871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8150.056.6896146
CGTATGC8300.056.088144
GTATGCC8550.054.03871545
GCACACG8950.052.40332811
ACGTCTG9200.050.9781415
TCGTATG9550.049.11097343
TACTCTA26550.047.5871434
CACACGT10000.046.89880412
GTACTCT27400.046.1108973
CTCGTAT9950.046.08029642
AGCACAC10400.045.76912710
GCCGTCT10400.045.4325949
CACGTCT10600.044.24518214
TCTAGTT28600.044.1782237
AGTCACT10800.043.10474428
CAGTCAC10850.042.9061127
ACTCTAG34100.041.8767365
GAGCACA11350.041.629869
CCAGTCA11450.040.35205526
CTCTAGT32500.039.630686
Produced by FastQC (version 0.11.5)