FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_5R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_5R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162977
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA111870.5172038352696308No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT58120.268703735638428No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA50540.23365944251834392No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG46570.2153051095781416No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT38220.17670090805403849No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT37900.17522146560042018No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA37170.17184648750310336No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA33780.15617364401008424No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA33310.15400071290633233No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC27700.1280642373913361No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA26980.12473549187069488No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG26170.12099065315997348No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA24370.11266878935837044No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT23830.11017223021788951No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT23470.10850785745756891No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT22320.10319111113987806No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA21780.10069455199939713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2150.047.2112746
CGTATGC2250.046.6686144
TACTCTA26500.046.358284
GTACTCT26400.046.2687233
GTATGCC2200.046.1393545
TCTAGTT27650.044.55477
TAGTTGT32700.037.460739
TCGTATG2750.036.91062543
ACGTCTG2850.036.84193815
TTGTACT34250.035.662421
TGTACTC34750.035.2500082
ACCTACT12550.035.13765333
ACACCTA12650.034.5864231
GCACACG3050.034.42527811
CTCGTAT2950.034.4066242
TGTTACC35550.034.0645413
GTTACCT36650.033.0421414
TACCTCT36700.032.90099716
ACTCTAG41700.032.6497275
CTCTAGT40250.031.4781156
Produced by FastQC (version 0.11.5)