FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_5G-ABF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_5G-ABF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282444
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC101670.4454435683854675TruSeq Adapter, Index 6 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA62900.27558178864410254No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG29360.12863404315724725No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA29150.12771397677226692No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT28280.12390227317734849No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA24870.1089621475926682No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT23510.10300362243279573No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA23420.10260930826780415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11550.065.1581446
CGTATGC11900.063.5358744
GTATGCC11850.063.5085745
ACGTCTG12550.060.51480515
AGTCACG12750.059.57207528
TCGTATG12750.059.3001543
GCACACG12850.058.8296511
CACGTCT13150.058.01981414
CTCGTAT13100.057.97918742
GCCGTCT13200.057.01087649
CATCTCG13450.056.4679639
CAGTCAC13500.056.0044727
AGCACAC13550.055.79047810
CACACGT13750.055.48804512
ACGACAT13800.054.7797835
GACATCT14200.053.23668737
GAGCACA14250.053.0487339
GACGACA14450.052.5578434
TCCAGTC14500.052.38348825
CCAGTCA15050.050.7017226