Basic Statistics
Measure | Value |
---|---|
Filename | HVWNMBGXB_n01_5G-ABF3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2282444 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC | 10167 | 0.4454435683854675 | TruSeq Adapter, Index 6 (97% over 36bp) |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 6290 | 0.27558178864410254 | No Hit |
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG | 2936 | 0.12863404315724725 | No Hit |
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA | 2915 | 0.12771397677226692 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 2828 | 0.12390227317734849 | No Hit |
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 2487 | 0.1089621475926682 | No Hit |
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT | 2351 | 0.10300362243279573 | No Hit |
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA | 2342 | 0.10260930826780415 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 1155 | 0.0 | 65.15814 | 46 |
CGTATGC | 1190 | 0.0 | 63.53587 | 44 |
GTATGCC | 1185 | 0.0 | 63.50857 | 45 |
ACGTCTG | 1255 | 0.0 | 60.514805 | 15 |
AGTCACG | 1275 | 0.0 | 59.572075 | 28 |
TCGTATG | 1275 | 0.0 | 59.30015 | 43 |
GCACACG | 1285 | 0.0 | 58.82965 | 11 |
CACGTCT | 1315 | 0.0 | 58.019814 | 14 |
CTCGTAT | 1310 | 0.0 | 57.979187 | 42 |
GCCGTCT | 1320 | 0.0 | 57.010876 | 49 |
CATCTCG | 1345 | 0.0 | 56.46796 | 39 |
CAGTCAC | 1350 | 0.0 | 56.00447 | 27 |
AGCACAC | 1355 | 0.0 | 55.790478 | 10 |
CACACGT | 1375 | 0.0 | 55.488045 | 12 |
ACGACAT | 1380 | 0.0 | 54.77978 | 35 |
GACATCT | 1420 | 0.0 | 53.236687 | 37 |
GAGCACA | 1425 | 0.0 | 53.048733 | 9 |
GACGACA | 1445 | 0.0 | 52.55784 | 34 |
TCCAGTC | 1450 | 0.0 | 52.383488 | 25 |
CCAGTCA | 1505 | 0.0 | 50.70172 | 26 |