FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_4G-ZAT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_4G-ZAT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485459
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT112610.32308513742379413TruSeq Adapter, Index 19 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA56290.16149953277315843No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT40710.11679953773663669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC15700.055.7331846
GTATGCC16100.054.7840847
TATGCCG16050.054.73588648
GCACACG17000.051.46827711
AGTCACG17050.051.11720728
GTCACGT17250.050.93036329
CTCGTAT16850.050.47830644
TCGTATG17650.049.97301545
ACGTGAT17600.049.91539832
AGCACAC18500.048.0512110
ACGTCTG18550.047.54435315
CACGTGA18650.046.91747331
TCACGTG18950.046.72881730
CACACGT19250.046.17974512
GCCGTCT19700.044.7714951
TCCAGTC19800.044.0182325
GAGCACA20300.043.6174779
CACGTCT20100.043.53038814
CAGTCAC20450.042.7908927
CCAGTCA21400.041.05486326
Produced by FastQC (version 0.11.5)