FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_3G-ABF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_3G-ABF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217423
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA78210.3527067230744878No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA43300.19527171856700323No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG36750.16573292511171753No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC35840.1616290622041893TruSeq Adapter, Index 2 (97% over 37bp)
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT33170.14958805784913387No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT33040.149001791719487No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA29410.13263143748396222No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA27130.12234923151784752No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA25460.11481796662161436No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA25020.11283368125973257No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT23530.10611416946608744No Hit
GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCT22530.10160443000726518No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA22370.10088287169385364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC5200.053.17876445
CGTATGC5400.051.85739544
CTCGTAT5550.049.8161642
ACGTCTG5700.049.73431415
TATGCCG5650.048.9432946
GCACACG5800.048.87792611
TCACCTA6050.046.8602930
CACGTCT6050.046.8571214
CATCTCG6050.046.27968239
CACCTAT6150.046.09729431
AGCACAC6150.046.09625210
TCGTATG6150.045.53024343
CACACGT6400.044.2946212
GCCGTCT6450.043.41549749
CAGTCAC6700.043.36284327
GTCACCT6600.042.95623429
AGTCACC7000.041.50163328
TCCAGTC7000.041.00253725
CCAGTCA7100.039.9338526
GAGCACA7250.039.585989
Produced by FastQC (version 0.11.5)