FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_2R-MYB44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_2R-MYB44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415582
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG49450.20471257030396814No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA45040.18645610043459504No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT30610.126718943923245No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC27490.11380280197484498No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG27240.11276785470333858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4350.037.0135344
TACTCTA15500.034.7732244
GTACTCT15450.034.6592333
ACTCTAG23100.033.635435
GTATGCC4700.033.51109345
TGTACTC17250.031.4471022
TCTAGTT17550.031.309677
CTCTAGT18000.030.9164476
TATGCCG5000.030.8004246
CTAGTTG17700.030.4511328
TTGTACT17600.030.2258071
TGCCGTC5250.029.33372948
TAGTTGT20700.026.3760789
GTTACCT20150.026.22593114
ATGCCGT5950.025.88270647
ACCTCTA20500.025.77977217
ATGCTGG21150.024.8240624
GCACACG6500.024.76751511
GCTGACT9050.024.7501811
TACCTCT21500.024.58020616
Produced by FastQC (version 0.11.5)