FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_2G-HB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_2G-HB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196930
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA76060.3462103935946981No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA36220.16486642724165085No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG33740.15357794740842903No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA29310.13341344512569814No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT29180.13282171029573084No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT28800.13109202386967267No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC28080.12781472327293086TruSeq Adapter, Index 3 (97% over 37bp)
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA27470.12503812137846906No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT27000.12289877237781814No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA26230.11939388146185814No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA25270.11502414733286905No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT23120.10523776360648723No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT22740.10350807718042905No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG22120.1006859572221236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTAG4000.049.8788131
CGTATGC4000.049.8754144
CTCGTAT4050.049.25853742
ACCTAGC4200.047.5036332
GTATGCC4550.045.38498345
TACTCTA18700.044.357644
GCACACG4500.044.33066611
TATGCCG4650.043.6562746
GTACTCT19250.042.9074863
TCACCTA4900.040.71739630
ACGTCTG5000.039.89760215
GCTATCT5100.039.8033437
TCTAGTT20800.039.7109577
TCGTATG5050.039.5043743
CCAGTCA5350.039.25533726
CACGTCT5200.039.0361114
AGCACAC5250.038.66522210
CACACGT5250.038.6643412
GTCACCT5350.037.2934229
TCCAGTC5650.037.17098225
Produced by FastQC (version 0.11.5)