FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_1R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_1R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3806835
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA73670.1935203390743229No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC55380.14547517819921274TruSeq Adapter, Index 7 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG42550.11177264052684185No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT40430.10620370990599803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7450.046.04282446
CGTATGC7700.045.00427244
GTATGCC7950.043.58847445
TACTCTA24200.041.0728844
GTACTCT24500.040.569953
ACGTCTG8750.040.39799515
TCGTATG8950.039.1098643
GCACACG9250.038.21482511
CTCGTAT9100.038.07953642
TCTAGTT27250.036.34737
AGCACAC10000.036.0486910
ATGCCGT9600.035.7316247
CACACGT10300.033.978912
GCCGTCT10550.033.50897249
CACGTCT10550.033.17371414
CAGTCAC10600.032.69006727
TCCAGTC11600.031.07890525
ACCTACT11550.030.90796733
TAGTTGT32350.030.6175259
GAGCACA11900.030.2934119
Produced by FastQC (version 0.11.5)