FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_1G-ABF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_1G-ABF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731928
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA66680.38500445746012535No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT34990.20202918366121456No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA32270.1863241428050127No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG30760.17760553556498884No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT25460.14700380154371312No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC23230.1341279776064594TruSeq Adapter, Index 7 (97% over 37bp)
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA22400.12933563058048603No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA22100.12760345695664024No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT21460.1239081532257692No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA18700.10797215588638788No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA17890.10329528710200425No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC17380.10035059194146638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC3200.052.50324645
CGTATGC3600.046.6695544
TATGCCG3850.043.63780646
GTACTCT19450.043.5477373
TACTCTA19550.043.3249864
GCACACG3850.042.72991611
TCTAGTT21400.040.398537
ACGTCTG4350.037.81733715
CACGTCT4450.036.9675114
CACACGT4500.036.55676312
GCCGTCT4650.036.1291849
CTCGTAT4750.036.10645742
CGGAATA4700.035.74482734
TCGTATG4800.035.73137743
AGCACAC4900.033.57156810
ACTCTAG28350.032.964055
ACCTACT9000.032.27604733
CTCTAGT27150.031.842676
CAGTCAC5200.031.63565827
TATCTCG5350.031.4038139