FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_12G-ZAT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_12G-ZAT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071255
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA76970.37161044873760113No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC52990.2558352303313691TruSeq Adapter, Index 10 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT37970.18331880912779933No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG36470.1760768229889608No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA30000.1448397227767706No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT27100.13083854957501612No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT23910.11543725905308617No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA23880.11529241933030941No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT22990.11099550755459854No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG21290.10278792326391488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7300.052.2623144
GTATGCC7400.051.55606545
TATGCCG7700.050.0019546
GCACACG8100.048.39227311
TCGTATG8350.046.52763743
CTCGTAT8300.045.54284342
AGCACAC8700.044.652610
ACTCGGC8550.044.6194732
TCACTCG8650.044.10576230
ACACGTC8900.043.64917413
ACGTCTG8900.043.64917415
CACACGT9100.042.68984612
TACTCTA18700.041.1750954
GTCACTC9200.041.0885629
CACGTCT9550.040.67828414
GAGCACA9600.040.46749
CGGCAAT9650.040.25966335
GCCGTCT9550.039.94823549
GTACTCT19500.038.7679333
TCCAGTC10250.038.5859425