FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_11R-GBF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_11R-GBF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215964
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA49910.22522929072854975No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA27000.12184313463576124No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC25290.11412640277549636TruSeq Adapter, Index 2 (97% over 36bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG23450.10582301878550374No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT23250.10492047704746105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT15150.039.500853
TATGCCG4000.039.37713246
CGTATGC4000.038.50295344
GCACACG4000.038.49513611
TACTCTA15800.037.8758164
GTATGCC4350.036.2096745
TCTAGTT17050.035.714777
CACGTCT4850.031.74856614
ACGTCTG5150.031.25818615
TCGTATG5200.030.96391343
CACACGT5100.030.19226312
ACTCTAG23850.030.0807365
AGCACAC5450.028.89543310
TGTACTC20600.028.7099652
ACCTACT7700.028.63468433
CTCGTAT5400.028.5181342
CTCTAGT21850.028.349466
TTGTACT21250.028.1617831
GTTACCT22050.027.29813814
TACCTCT21900.027.00693716
Produced by FastQC (version 0.11.5)