FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_11G-ABF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_11G-ABF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983281
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA79000.3983298382831278No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA40470.20405580449769853No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT38310.19316476081805856No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC36770.18539985004646342TruSeq Adapter, Index 10 (97% over 36bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG34950.1762231373163964No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT32950.1661388376130261No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA26930.13578509550588141No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT26500.13361697106965678No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA25890.13054125966012886No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA23870.12035611695972483No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG20300.1023556419892088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5000.053.90504546
CGTATGC5000.053.2049844
GTATGCC5350.051.03281445
CTCGTAT5400.049.90704342
TCGTATG5550.048.56310743
GCACACG5800.045.8559611
ACGTCTG5800.045.8559615
AGCACAC5900.045.0787410
CACGTCT6100.043.6007514
CACACGT6200.042.8975112
GCCGTCT6600.040.835149
GTACTCT22650.040.7933773
TACTCTA22950.040.7176364
TCACTCG6650.039.9997130
TCCAGTC6700.039.18277425
GAGCACA7000.038.4977849
CAGTCAC6950.038.27627
AGTCACT6950.038.27503628
TCTAGTT24750.037.8968057
CCAGTCA7050.037.7330826