FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_10R-MYB44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_10R-MYB44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899224
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA98450.518369607797711No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT47890.25215561724156815No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA47800.2516817394893915No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG45010.23699152917191443No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT37140.19555355239824265No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT36950.1945531438103141No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG35460.18670783435761132No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA32880.1731233387952132No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA32600.17164905245510798No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA30440.1602759864028677No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC27000.14216332565300355No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG25690.1352657717046541No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT25370.13358087303024815No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC24570.1293686263442332TruSeq Adapter, Index 9 (97% over 36bp)
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC22720.11962780588282372No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG20710.10904453608421123No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA19650.10346330922524148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT25700.046.026681
TACTCTA26200.045.683814
TGTACTC26350.045.1569252
GTACTCT27400.043.9385153
ACTCTAG31900.043.554935
CTCTAGT28450.042.808946
TCTAGTT28550.042.0471847
GTATGCC4650.039.89281545
CGTATGC4900.039.28812444
GCACACG5000.038.5003311
CTAGTTG31900.036.8635838
TATGCCG5050.036.7329946
ACGTCTG5150.036.69838315
AGCACAC5600.034.3734910
TAGTTGT34600.033.9878359
CACGTCT5950.032.35236714
ACCTACT12650.031.81510533
TGTTACC37400.030.97620613
TATCTCG5900.030.85097339
GTTACCT37700.030.63606514
Produced by FastQC (version 0.11.5)