FastQCFastQC Report
Wed 14 Aug 2019
HVWNMBGXB_n01_10G-HB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWNMBGXB_n01_10G-HB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056864
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA118120.5742722902437886No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG55750.2710436859218694No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC52600.2557291099460149TruSeq Adapter, Index 6 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT48580.2361847939387339No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA39990.19442218834108624No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA37620.18289979308306237No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT36800.17891314155918914No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT35090.1705995146008681No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG34830.16933545436159125No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC29980.14575586912892638No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA26850.13053852855609316No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA25470.12382928574762356No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA23640.11493224637117476No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA20890.10156237845574621No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT20650.10039555361949064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC7350.061.90826445
CGTATGC7500.060.67010544
TATGCCG7650.059.4804946
TCGTATG7750.059.1646443
CTCGTAT8050.056.9583642
GCACACG8450.054.25551211
AGCACAC8900.051.51225710
CAGTCAC8950.050.84210627
GTCACTC8950.050.8408729
GCCGTCT8950.050.8408749
TACTCTA24100.050.5373654
ACGTCTG9200.050.2129115
GTACTCT24400.049.9160043
AGTCACT9150.049.72958828
CACGTCT9350.049.40735214
CACTCCC9250.049.19197531
TCCAGTC9550.048.3785325
GAGCACA9600.047.7584769
CACACGT9650.047.5098712
TCTAGTT26450.046.3119167
Produced by FastQC (version 0.11.5)