FastQCFastQC Report
Tue 6 Aug 2019
HVWM5BGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWM5BGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465604711
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7635870.1639989849673149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT16182700.062.2001341
AGCAGTG16149650.062.123652
GCAGTGG18100200.055.344953
AGTGGTA19541150.051.2799225
CAGTGGT20694700.048.412114
GTGGTAT21735250.046.0822336
GGTATCA22544600.044.399718
TGGTATC22945400.043.6441967
GTATCAA22960150.043.5537729
ACGCAGA21962500.025.61107314-15
CAACGCA22169900.025.38909312-13
GCAGAGT22762450.024.74834416-17
AGTACAT22686050.024.70537220-21
TACATGG22726200.024.568122-23
AGAGTAC23127100.024.39190318-19
ATCAACG23457250.024.10414110-11
CATGGGG14210800.023.84138524-25
GTACATG21962650.022.75307822-23
CGCAGAG22399950.022.46904216-17
TATCAAC22590450.022.24518610-11
Produced by FastQC (version 0.11.5)