FastQCFastQC Report
Tue 6 Aug 2019
HVWM5BGXB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWM5BGXB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465604711
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAGC403321328.66231183816351No Hit
GCTACCTG400485238.60139986856791No Hit
CTGTAACT397269478.532333557080353No Hit
TGCTGTAA388215748.337882560642734No Hit
TCTAGCGA376606098.08853693063256No Hit
CTGGACTC365927087.859179070892177No Hit
GACCCTAC364283227.823873156644241No Hit
AAAGTGCT349773167.512234127716977No Hit
TGTAGAAG346862887.449728746408669No Hit
GCCTTGGT330835457.105500485367726No Hit
AAACCTCA302800286.503376637870831No Hit
AGAGTGTG274982395.905919409823154No Hit
GGGGGGGG182971563.9297617845623556No Hit
GCCCCTAC7670660.16474618531082688No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)