FastQCFastQC Report
Tue 6 Aug 2019
HVWM5BGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWM5BGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465604711
Sequences flagged as poor quality0
Sequence length29
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGAA2473150.020.4181821
AACGAAG1995950.020.3800682
CGGCCCA1807700.020.236249
GCACGCA2472700.020.1884063
AATGCGT3180100.020.1834813
GACCGTG1951050.020.109741
GGCGCAA1658700.020.0024154
GCGCAAC1655500.019.989615
GGCCCAA1834250.019.94011910
CGCAGCT2362600.019.848296
ACGCAGC2302800.019.8428945
CCGATGG2216300.019.8282661
ACCGTGA1791400.019.7271272
CGTCGGC1883950.019.718526
GAATGCG2179300.019.7161032
GCATCGA2536650.019.6129577
AGCGACC1257850.019.4835229
GCAGCTC2353600.019.4727067
ATCGAGC2204850.019.4160049
ACGAAGT2110900.019.269633
Produced by FastQC (version 0.11.5)