FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28661434
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4206571.467676041610479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG158700.013.4705455
TACACTG121950.012.5213825
CTACACT108250.012.3246114
TAGACTG94050.011.9062985
TAGGACA93150.011.8962694
GTATAGA136450.011.8239551
TACACAG170900.011.6154325
ACTGATC101800.011.6032388
GTCCTAG59800.011.3140151
CTAGCCT83550.011.2562384
GTAGGAC51450.011.1705183
CTATACT128250.010.9710944
TCTATAC130650.010.9409853
GTGTTAG112700.010.8192331
TGCACTG126100.010.7253795
CAGGACT105750.010.685664
TAGACAG170900.010.6368435
GTATAGG70400.010.5609721
GTGCTAG70550.010.5385171
CTAAGAC101600.010.5157053
Produced by FastQC (version 0.11.5)