FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_9361.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_9361.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20963168
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG267300.015.7562715
GCCGACC26600.014.450916145
GTATAGG85250.014.3036461
TACACAG239400.013.6561625
CTATACT159700.013.6173834
TACACTG191550.013.4723745
CTACACT137550.013.3332874
CTTACAC133900.013.3189243
GTATAGA163950.012.8828941
GTGTAGG97200.012.69446851
ATACTGT337750.012.469736
TCTACAC131450.012.3538423
CCTACAC96300.012.3461683
GTCCTAG98350.012.1770321
GTGTTAG163000.012.1564541
CTAGTAC65750.012.0183643
GTGTAAG144550.012.000841
CCTATAC82450.011.8701923
TGCACTG219950.011.8646485
ACGAACG10555.493348E-1011.686853145
Produced by FastQC (version 0.11.5)