FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_9109.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_9109.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19899604
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG225810.11347461989695876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG203250.017.3337045
CTATACT138200.015.4215954
CCTACAC91950.015.3750123
TCTATAC138000.015.0777093
TACACCG54900.015.0528315
TACACTG144500.014.7992535
TATACCG33650.014.2181975
GTATAGG77650.014.2085621
CTACACT114450.014.0613564
GTGTAGG94350.013.4630711
TACACAG176500.013.389325
GTACTAG64600.013.0338721
GCTCGGA50150.012.7203016
GTGTAAG105850.012.6861221
GTATAGA141000.012.612321
GTCCTAG74400.012.5853341
ATACTGT268400.012.50501256
CGGAGAT62150.012.4806159
GTATTAG151250.012.4294631
ACTGTAC171250.012.1491158
Produced by FastQC (version 0.11.5)