FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_8337.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_8337.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33201087
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC106450.028.751694145
CCGTATA93200.023.423258145
CGCCGTT54900.021.79758145
ACGAACA173300.019.251118145
CGGTGGG192150.018.9856145
TCTCGGG112000.018.066868145
GCCGTAA72400.017.4304145
ACGAACG52450.017.00812145
TGACGCG51150.016.873222145
TATACTG317900.016.2129925
TACACTG265800.015.2182015
CTACACT188950.015.1159254
GCTCGGA47500.014.95603756
TATACCG28400.014.8044995
GTGTAGG146200.014.7959211
TACACCG34850.014.5606015
TCGGAGA57950.014.3858128
CGAACTA104950.014.304893145
GTACTAG95650.014.0397651
CCTACAC139150.013.9631073
Produced by FastQC (version 0.11.5)