FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_8337.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_8337.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19423774
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG205750.015.8545975
TACACTG148750.015.1560485
TATACCG40600.014.9980865
CTATACT146650.014.6811964
TACACCG48400.014.6778785
CTACACT118750.014.1625484
GTATAAG149150.013.38186551
GTGTAGG92750.012.9115161
GTATAGA155200.012.9069791
GGACCGT28200.012.8529696
TCTATAC152350.012.80073
ATACTGT263950.012.7706756
CCTATAC81250.012.7595873
GTATTAG152550.012.6078451
TCTACAC127550.012.5613343
GTATAGG80600.012.5166171
GTCTTAG103800.012.37608051
CTAGGAC66850.012.1462033
GTCCTAG71150.012.1388991
TAGACTG113000.011.9962575
Produced by FastQC (version 0.11.5)