FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_8336.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_8336.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18719500
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195790.10459146878923048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC57550.027.094343145
GCCGACC38200.023.921734145
GCTCGGA24400.020.7966846
TCGGAGA30850.018.3286298
CCGTATA34100.018.077986145
CGGAGAT35650.016.470859
GACGACA49800.016.019493145
CGCCGTT18150.015.583802145
TATACTG210150.014.5913975
CCTACAC83250.014.1950453
TACACTG160350.014.0145325
CTATACT130600.013.5990934
GCCGTAA27750.013.5901985145
GTGTAGG81950.013.5504271
TAACACG53700.012.8243794
GTATAGG64250.012.76485351
CCTATAC67750.012.6271733
CTTACAC113750.012.6196483
GTCCTAG83650.012.5809261
CTACACT124350.012.4754164
Produced by FastQC (version 0.11.5)