FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_7651.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_7651.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19322112
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG259000.016.1215275
TACACTG178250.014.803195
CTATACT156850.014.558264
TACACAG222300.014.022085
CTACACT133550.013.5157114
CCTATAC77500.013.4707393
CCTACAC92350.013.3457393
TCTATAC148050.013.270633
ATACTGT312900.013.2517716
TATACCG17850.012.9955665
GTATAGA155850.012.900861
TAGACTG141150.012.8906935
TACACCG18750.012.7583975
GTGTTAG148450.012.7127311
GTATTAG146000.012.6774831
TCTACAC129050.012.6402353
GTATACG19650.012.5592031
TACAGGT271150.012.4315924
ATACCGT28100.012.3827856
CTAGGAC88000.012.2753613
Produced by FastQC (version 0.11.5)