FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_7431.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_7431.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18466510
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG254050.015.8936925
CCTATAC75500.013.9237343
GTGTAGG82850.013.6660981
GTATAGA147100.013.3711591
CTATACT150200.013.2243444
GTATTAG133950.013.0583031
GTATAGG74500.012.8596761
ATACTGT312750.012.5861046
TACACTG164750.012.540315
TATACCG18600.012.4717055
TCTATAC145700.012.390093
GTGTAAG123850.012.1893261
ACAGGCG165750.012.0710315
CTAGGAC85900.012.0691743
CTAGACT104900.011.95544
GTACTAG62650.011.9324221
ACTGTAC228900.011.9077828
CTACACT123400.011.8666944
GTGCTAG96500.011.7330171
TCTACAC117600.011.52839853
Produced by FastQC (version 0.11.5)