FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_6751.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_6751.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23497699
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC75150.027.409409145
CCGACGC124750.023.255718145
CCGTATA59550.022.166643145
GACGACA90250.020.814352145
CGCCGTT39150.018.896385145
CGACTAT47000.018.363668145
GCCGTAA51750.015.977353145
TCTCGGG71150.015.698428145
TATACTG232350.015.537125
CGGTGGG113550.015.138105145
GCTCGGA36500.014.2995876
TACACTG187450.014.154015
CTATACT160550.014.0422214
GTGTAAG144200.013.84121
TACACCG25750.013.7943965
CGGAGAT44750.013.7694389
CTGCCGC267850.013.593222145
CTACACT137750.013.5773134
TCGGAGA45850.013.4391198
TCTACAC137050.013.1719863
Produced by FastQC (version 0.11.5)