FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_6215.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_6215.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21420257
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC36100.016.474842145
TATACTG283050.016.4419465
TACACTG189500.013.8860515
TCTATAC163250.013.8110633
CTATACT176850.013.690644
ATACTGT348950.013.2745266
GTATAGG85650.012.9670111
TACACAG236850.012.8545615
TAACACG59600.012.7710154
CTTACAC135200.012.440313
CTACACT144500.012.3911464
TCTACAC137100.012.3207853
GTGTAGG99050.012.3120571
GTATAGA171100.012.3033631
GTACTAG76850.012.2793481
GTGTAAG142950.012.1871711
GTCCTAC64650.012.1263771
GTCCTAG92800.012.124381
GTATTAG168600.012.0552511
CCTACAC97150.012.0144243
Produced by FastQC (version 0.11.5)