FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_6215.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_6215.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21592020
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221320.10250083132564715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG215650.014.9586695
TACACCG65600.014.6970515
TACACTG142700.014.5794225
CTACACT124200.013.8911694
CTATACT150400.013.5920414
TCTATAC156300.013.5439853
GTATTAG171400.013.3804241
GTCTAGG74400.013.2665971
CTTACAC121800.012.9757323
GTCCTAG68750.012.8789581
CTAGGAC65300.012.8785643
CGGTGGG109700.012.627675145
TCTCGGG73150.012.591749145
CCTACAC103750.012.5778673
GTATAGA159300.012.57438851
GCCGACC111250.012.451738145
CGGAGAT74300.012.2934329
TATACCG41450.012.2420995
TAGACTG118100.012.2147775
TACACAG178000.012.0953615
Produced by FastQC (version 0.11.5)