FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_6119.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_6119.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26447952
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCC63600.027.93868145
GCCGACC58350.026.22639145
CGGAGAT47900.020.582399
CGACGAA77050.020.331903145
CCGTATA54150.020.224388145
TCGGAGA46200.019.7706538
ATCGCAA93300.018.656343145
TCTCGGG71000.017.774107145
GCCGTAA46500.017.624727145
CGCCGTT31200.017.434263145
GCTCGGA39050.017.078426
CGGTGGG116300.017.024715145
TGACGCG26850.017.017399145
TATACTG295550.015.599375
GTATAGG99100.015.5276571
TACACTG209700.015.1756415
CTATACT180500.015.1408424
CTACACT159250.015.0672654
CTGCCGC315450.014.438631145
GTACTAG78850.013.9001321
Produced by FastQC (version 0.11.5)