FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_4340.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_4340.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16868192
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG142400.016.9520135
TACACTG90050.015.2952615
TACACAG108850.014.3850815
CTATACT121600.014.0690724
TACACCG94850.013.6040965
GTGTAAG90300.013.5845831
TATACCG66800.013.4565045
TCTACAC114950.013.1814833
TCTATAC136150.013.1524373
GTATAAG127050.013.1401561
TATACAG147000.013.1175085
GTATAGG88150.012.7631171
GTGTAGG93750.012.4652781
GTATAGA139000.012.4282371
CTTACAC89200.012.3539453
CTACACT87600.012.33017354
GTCTAAG65400.012.20851
TTATACT204500.012.1941684
TTACACT118500.012.17367654
TAGACAG125550.012.067485
Produced by FastQC (version 0.11.5)