FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_4329.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_4329.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18127303
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG199150.016.0525025
TACACCG41450.015.3901415
TACACTG140800.015.2910815
CCGACGC120500.014.445186145
CTACACT107650.014.0740114
GTCCTAC58200.013.84256651
GTGTAGG91500.013.7227661
CTATACT126650.013.6225194
CCTACAC87000.013.0828733
TCTATAC129550.013.0389093
TACACAG171750.012.9154375
TATACCG28650.012.9041665
GTCCTAG75850.012.8222971
GTATAGG75700.012.7518261
GTGTAAG113750.012.569891
GTATAGA133600.012.3863981
CTTACAC108100.012.2058623
TAGTACT98000.012.0572194
CTAGGAC71100.012.0319273
TATACAG172100.012.004625
Produced by FastQC (version 0.11.5)