FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_341.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_341.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18147000
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC55100.020.534294145
CGGAGAT37700.017.4980729
GCCGACC30850.017.39736145
GACGACA41000.016.097717145
GCTCGGA26750.015.717596
TACACTG150650.014.9167545
TATACTG222850.014.5729395
TCGGAGA38600.014.0852758
CTACACT117300.013.6577274
CTAGGAC77400.013.5820793
GTGTAAG120700.013.5302381
GTATAGG73050.013.5129091
CTATACT141050.013.3110054
ATACTGT276650.013.0228936
CTTACAC116250.012.9097143
CCTACAC85900.012.6601053
GTATAGA140750.012.5826341
GTCTAGG83450.012.5246711
GTGTAGG84500.012.1972461
GTATTAG136300.012.1414111
Produced by FastQC (version 0.11.5)