FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_3256.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_3256.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18880292
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC26900.017.254784145
TATACTG240900.015.7379745
TACACTG167300.015.5554455
CTACACT126300.014.0046274
CCTACAC86750.013.9564753
CTATACT148700.013.9424834
ATACTGT300100.013.5512946
GTGTAGG87100.013.4175741
GTGTACG19300.013.1636711
CGGAGAT35000.013.0485129
GTCCTAC55100.012.9104311
TCTATAC148200.012.8657863
GTGTAAG131400.012.7609461
TACACCG17700.012.6961595
TAGACTG133300.012.56220155
TACACAG220400.012.5313345
TATACCG17500.012.4270245
TACAGGT257650.012.2952234
GTCCTAG87150.012.24381
CTTACAC124300.012.1900073
Produced by FastQC (version 0.11.5)