FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_30982.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_30982.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16797593
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG130150.016.2081765
CCTACAC98200.014.913143
TACACCG110800.014.6552615
TACACTG84250.014.5412625
CTACACT87550.014.3244474
GTGTAGG93200.013.8617621
GGACCGT47850.013.7862056
CTATACT112900.013.3554124
TAGACAG96150.013.3447145
TCTACAC121750.013.338543
TATACAG149650.013.0789345
CTACACC157400.013.0337574
TAGACTG65250.012.9984235
TACACAG99050.012.9540065
GTATAGG82600.012.8288351
TAGGACT76650.012.6730324
TTATACT199600.012.2034
GTATTAG148200.012.1945411
TCTATAC124850.012.0201823
GTATATA223800.011.869581
Produced by FastQC (version 0.11.5)