FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_30978.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_30978.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19736239
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG146450.015.6911145
TATACTG197650.015.2573955
GTATAGG93950.013.59751
GTGTAGG99400.013.4361431
CTACACT121850.013.3857944
TATACCG44700.013.298125
CTATACT147650.013.2561524
GTATTAG156750.013.10454751
TACACCG67950.013.0153025
CCTATAC87650.012.5725663
GTATAGA166500.012.4679471
TATACAG195600.012.3783155
TAGACTG111500.012.2877065
CCTACAC103450.012.2642173
TCTATAC154300.012.1223293
TCTACAC143050.012.1127383
CTAGGAC62450.012.0735013
GTAGGAC69350.012.0221933
CTAGTAC55850.011.9425473
TAGGACT108850.011.9209754
Produced by FastQC (version 0.11.5)