FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_30976.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_30976.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23269336
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC121100.020.24204145
CCGTATA67850.018.27797145
GCCGACC101400.018.238283145
TATACTG238850.015.8427315
GACGACA107800.015.809962145
CTATACT151350.015.714
TATACCG35950.015.3249655
TACACTG170150.014.7410665
CTACACT128600.014.5433194
TCTCGGG68000.014.398146145
CGGAGAT58950.014.141799
TACACCG50150.013.8766645
GTATAGA165650.013.7140351
GTATAGG88500.013.6957421
CCTACAC99550.013.6184623
GTGTAGG106350.013.5808811
TCTATAC155300.013.5379923
CGGTGGG113600.013.279041145
GTCCTAG84850.013.0873571
GTGTAAG135450.012.9136911
Produced by FastQC (version 0.11.5)