FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_30908.TR4_batch_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_30908.TR4_batch_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11644426
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118120.10143909197413423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC31900.022.73596145
GACGACA34500.020.602076145
CCGACGC73800.020.048311145
TATACTG146350.015.9990315
CGCCGTT18400.015.372722145
TACACTG98500.015.3077365
CTATACT88200.015.1228324
CTACACT73450.014.014594
TACACCG15550.013.9853955
GTATAGA86650.013.9046741
TATACCG12000.013.8941025
TAGGACT66400.013.8649884
TCTATAC84800.013.8491643
GTGTAGG53750.013.6384241
GTGTACG13900.013.576251
TAGACTG76950.013.4713565
GCTACAC44800.013.4308813
TACACAG120000.013.410835
GTATAGG43550.013.3328531
TAGACAG147750.012.8545735
Produced by FastQC (version 0.11.5)