FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_30904.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_30904.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18751308
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG222900.015.740465
CTATACT145600.014.9362654
TCTATAC140350.014.7218383
TACACTG154300.014.6108875
GTGTAGG93100.014.5790071
GTGTAAG120600.013.6018251
TATACCG27950.013.4866365
CTAGGAC79400.012.9657443
CTACACT121950.012.7802344
GTATAGA141150.012.7014151
GTATTAG142250.012.6031961
CTTAGAC86650.012.2992433
GTCCTAG84550.012.1901981
GTCTTAG105700.012.1544211
TACACAG198100.012.0024945
GTATAGG81750.011.9862141
ATACTGT284000.011.971176
ATAGGAC83850.011.9317913
CTAGACT101600.011.91529754
CCTACAC91650.011.8655643
Produced by FastQC (version 0.11.5)