FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_30891.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_30891.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21700488
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG103850.015.91535
CTATACT141100.015.5155594
TACACCG116600.015.3562415
CTACACT101050.015.3519994
TATACTG152600.014.7738065
TAGACTG80750.013.645445
TATACCG70250.013.62116055
GTATAGG92700.013.46611
TACACAG122350.013.2718225
TCTATAC149350.013.2031383
GTATAGA149550.013.1029921
TAGACAG120450.013.0598875
GTATAAG149000.012.9078161
TCTACAC139200.012.7076173
CTTACAC98100.012.6369023
GTGTAGG116650.012.6300311
CCTACAC117550.012.581173
TTACACT137750.012.3669824
CTAGACT55250.012.3334064
TATACAG172850.012.2042225
Produced by FastQC (version 0.11.5)