FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_30890.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_30890.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17594456
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG133250.015.6678445
CTACACT93150.014.0857414
TACACTG89800.013.9654125
TATACCG69900.013.7929915
GTATAGA144950.013.217711
TACACCG98250.013.2070075
TACACAG113350.013.1743645
GTGTAGG105950.012.9458731
CCTACAC104850.012.7224763
TATACAG157500.012.7031815
GTATAGG96050.012.5424171
CTAGACT58350.012.2992414
GTGTAAG95750.012.2785411
TAGACTG72800.012.2477885
CTATACT128650.012.1146994
TAGACCG56900.011.9756625
CTACCCT70350.011.9530264
CCTATAC94900.011.84098153
TCTATAC136800.011.8179183
TCTACAC134100.011.7855523
Produced by FastQC (version 0.11.5)