FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_30889.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_30889.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19697164
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG185700.015.185095
TACACTG131450.014.5587135
CTACACT119050.014.0657654
TACACCG59600.013.9872155
GTATAGG91050.013.9498131
CTATACT150950.013.4944124
CTAGGAC75950.013.3634143
GTGTAAG113800.013.201961
CCTACAC93850.012.4368033
GTATTAG160450.012.3942751
CCTATAC89700.012.3656283
GTGTAGG99700.012.1571451
TATACCG45700.012.0552745
CTAGACT92650.011.97094
GTACTAG65650.011.9398611
TAGACTG104050.011.9133445
GTATAGA154500.011.8850851
TCTATAC156050.011.8465963
ATACTGT245750.011.7990266
GCTCGGA44950.011.7726136
Produced by FastQC (version 0.11.5)