FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n02_MG_2397.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n02_MG_2397.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24456564
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG70600.015.9150835
TATACTG235000.015.5160815
TACACTG168050.015.3134045
CTATACT165300.014.9542694
CTACACT139100.014.4877644
TCTATAC175250.013.7342943
TACACAG208200.013.370035
GTATAGG102050.013.2296321
TCTACAC161750.013.2222093
GTGTAGG117000.013.2142211
GTCTTAG119700.012.9767951
CCGTATA82200.012.970203145
GTGTAAG139700.012.6777241
GTGTTAG154750.012.6642931
GTATAGA176550.012.6217191
CTTAGAC97600.012.55345253
TAGACAG225800.012.552635
GCCGACC122850.012.397821145
TAGTACT124250.012.19364554
GTATTAG171300.012.1187131
Produced by FastQC (version 0.11.5)